Antigen

Antigen Class

TFs and others

Antigen

KMT2A

Cell type

Cell type Class

Others

Cell type

Acute lymphoid leukemia

NA

NA

Sample

source_name

Leukemia cell line (SEM)

cell type

Bone marrow aspirate from a 15-year old MLL-AF4 ALL patient

chip antibody

Bethyl A300-086A

sirna

RUNX1 siRNA (Dharmacon J-003926-05)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (EZH2). For reference normalisation 10% S2 cells were spiked in prior to sonication. Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

21162014

Reads aligned (%)

92.9

Duplicates removed (%)

2.1

Number of peaks

10750 (qval < 1E-05)

hg19

Number of total reads

21162014

Reads aligned (%)

92.2

Duplicates removed (%)

2.2

Number of peaks

10610 (qval < 1E-05)